English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27287/39131
Visitors : 2445990      Online Users : 32
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39344

Title: Recovery of isoamylase from Pseudomonas amyloderamosa by adsorption-elution on raw starch
Authors: Tsuei-Yun Fang
Long-Liu Lin
Wen-Hwei Hsu
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: raw starch adsorption
Pseudomonas amyloderamosa
isoamylase
Date: 1994-03
Issue Date: 2016-12-07T01:41:37Z
Publisher: Enzyme and Microbial Technology
Abstract: Abstract: The isoamylase from Pseudomonas amyloderamosa WU2130 was adsorbed onto raw starch, and the adsorbed enzyme was eluted by maltose in 50 mm acetate buffer. The adsorption of isoamylase to adsorbent was affected by the sources of raw starch, temperature, and pH, whereas the temperature and elution time had no obvious effects on the elution of adsorbed isoamylase. Raw starch could be used repeatedly on the adsorption-elution cycle with good reproducibility. About 52.5% of isoamylase was recovered, and the eluted enzyme had a specific activity of 72,828 U mg−1 protein with a purification of 13.3-fold. These results suggest that the raw starch adsorption-elution technique has a great potential in recovery of isoamylase from culture broths.
Relation: 16(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39344
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML41View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback