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Title: High-performance liquid chromatographic determination for bile components in fish, chicken and duck
Authors: Yen-Hung Yeh
Deng-Fwu Hwang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Bile acids
Cyprinol sulfate
Date: 2001-02
Issue Date: 2016-11-23T02:38:52Z
Publisher: Journal of Chromatography B: Biomedical Sciences and Applications
Abstract: Abstract: A HPLC procedure for the determination of 13 bile acids and cyprinol sulfate in animals was developed. The mobile system 0.3% ammonium carbonate solution–acetonitrile (73:27, v/v) 10 min→(68:32) 10 min→(50:50) 10 min was available for separating all 14 bile components, except for deoxycholic and glycodeoxycholic acids, which could be further separated with 0.3% ammonium carbonate solution–acetonitrile (73:27). After applying this method, grass carp and common carp bile was found to contain mainly cyprinol sulfate, while the other 12 fish species bile contained mainly taurocholic, taurochenodeoxycholic and cholic acids. Chicken bile was mainly composed of glycolithocholic and taurocholic acids, but duck bile was mainly composed of taurochenodeoxycholic, cholic and ursodeoxycholic acids.
Relation: 751(1)
Appears in Collections:[Department of Food Science] Periodical Articles

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