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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39048

Title: Effect of processing on sequence of cytochrome b gene and its restriction site in the meat of puffer Takifugu rubripes
Authors: CHAO-AN CHENG
YU-WEN HSIEH
TAMAO NOGUCHI
OSAMU ARAKAWA
DENG-FWU HWANG
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Takifugu rubripes
processing
cytochrome b gene
polymerase chain reaction
puffer
Date: 2001-12
Issue Date: 2016-11-23T01:19:23Z
Publisher: Journal of Food and Drug Analysis
Abstract: ABSTRACT: The polymerase chain reaction (PCR) and direct sequence analysis were used to determine the amount of genetic variation in a 376-nucleotide region of the mitochondrial cytochrome b gene in fresh, frozen and steam sterilized meats of puffer Takifugu rubripes. A diversity of sequence between fresh, frozen and steam sterilized meats was not found. The puffer specimens collected from either culture ponds in Taiwan or wild seawaters and culture waters in Japan had the same sequence of 376-nucleotide region. Restriction enzyme BstZI was found to cut the amplified region of cytochrome b gene in fresh, frozen and steam sterilized meats of puffer T. rubripes, while restriction enzyme AatII did not. This indicates that the sequence and restriction site analyses might be used to authenticate species of different processed meats of puffer T. rubripes.
Relation: 9(4)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39048
Appears in Collections:[食品科學系] 期刊論文

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