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Title: Electrophoretic identification of muscle proteins in 7 puffer species
Authors: T.-Y. Chen;D.-F. Hwang
Contributors: 國立臺灣海洋大學:食品科學系
Date: 2002-04
Issue Date: 2016-11-22T07:53:52Z
Publisher: Journal of Food Science
Abstract: ABSTRACT: Species identification of puffer species using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie blue and Coomassie blue/silver double staining was performed. A comparison was also made among extracts of sarcoplasmic, myofibrillar, SDS-soluble, and urea-soluble proteins. Protein bands of lower molecular weight showed the species-specific characteristics. Double staining better discriminated the protein banding patterns of different puffer species than Coomassie blue staining. Species identification of all tested puffers could be achieved by judging from the SDS-PAGE patterns of the 4 protein extracts stained with both stains along with protein band densities.
Relation: 67(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/39037
Appears in Collections:[食品科學系] 期刊論文

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