English  |  正體中文  |  简体中文  |  Items with full text/Total items : 28611/40649
Visitors : 627359      Online Users : 81
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38929

Title: Preliminary study on puffer fish proteome-species identification of puffer fish by two-dimensional electrophoresis
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: protein
puffer fish
Two-dimensional electrophoresis
species identification
Date: 2004-04
Issue Date: 2016-11-16T02:37:50Z
Publisher: J. Agric. Food Chem
Abstract: Abstract: The aims of this work were to determine the differential characterization of the urea soluble protein components of puffer fish species and to establish a preliminary proteomic database using an immobilized pH gradient two-dimensional electrophoresis (2DE) technique. The puffer fish muscle proteins resolved into 171−260 spots in the 2DE gels, with a pI range of 3−10 and molecular mass range of 7.4−205.0 kDa, following Comassie blue staining. Puffer fish muscle proteins fell in the region with pI values of 3.5−7.0, and molecular masses of 7.4−45.0 kDa were well-resolved and were good for species comparison. The more acidic proteins of lower molecular masses showed species specific characteristics. Therefore, the species of puffer fish can be differentiated from the comparison of the characteristic 2DE protein patterns.
Relation: 52(8)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38929
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat

All items in NTOUR are protected by copyright, with all rights reserved.


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback