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|Title: ||Identification of toxin and species of causative fish roe implicated into a food poisoning incident|
|Authors: ||Ya-Jung Wu|
|Issue Date: ||2016-11-14T08:11:34Z
|Publisher: ||J. Fish. Soc. Taiwan|
|Abstract: ||ABSTRACT: The food poisoning incident due to ingesting fish roe occurred in Taiwan in March 2008. A total of 3 victims were reported. Symptoms featured paralysis, coma, nausea, vomiting and ataxia. Using the cytochrome b gene sequence and PCR-RFLP method identified the fish species of the roe. The results indicated that species of causative fish roe was Lagocephalus lunaris. Toxicity of causative fish roe was 425 ± 80 mouse units (MU) by a tetrodotoxin (TIX) bioassay. The toxin was partially purified from the acidic methanol extract of the roe by using a C18 solid phase extraction column. The eluate was then filtered through a 3,000 dalton cut-off ultrafree microcentrifuge filter. Analyses of high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) identified the purified toxin mainly containing TIX. It is concluded that the causative agent of the above food poisoning was TIX and the roe species was a toxic puffer L. lunaris. This food poisoning is a case of puffer intoxication.|
|Appears in Collections:||[食品科學系] 期刊論文|
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