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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38907

Title: Identification of toxin and species of causative fish roe implicated into a food poisoning incident
Authors: Ya-Jung Wu
Hsiao-Chin Jen
Jou-Fang Deng
Deng-Fwu Hwanq
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: PCR-RFLP
Lagocephalus lunaris
foodpoisoning
tetrodotoxin
LCIMS
Date: 2008-12
Issue Date: 2016-11-14T08:11:34Z
Publisher: J. Fish. Soc. Taiwan
Abstract: ABSTRACT: The food poisoning incident due to ingesting fish roe occurred in Taiwan in March 2008. A total of 3 victims were reported. Symptoms featured paralysis, coma, nausea, vomiting and ataxia. Using the cytochrome b gene sequence and PCR-RFLP method identified the fish species of the roe. The results indicated that species of causative fish roe was Lagocephalus lunaris. Toxicity of causative fish roe was 425 ± 80 mouse units (MU) by a tetrodotoxin (TIX) bioassay. The toxin was partially purified from the acidic methanol extract of the roe by using a C18 solid phase extraction column. The eluate was then filtered through a 3,000 dalton cut-off ultrafree microcentrifuge filter. Analyses of high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) identified the purified toxin mainly containing TIX. It is concluded that the causative agent of the above food poisoning was TIX and the roe species was a toxic puffer L. lunaris. This food poisoning is a case of puffer intoxication.
Relation: 35(4)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38907
Appears in Collections:[食品科學系] 期刊論文

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