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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38906

Title: Occurrence of tetrodotoxin in two gastropods from Philippine by using HPLC and LC-MS/MS methods
Authors: Hsiao-Chin Jen
Deng-Fwu Hwang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: LC-MS/MS
Date: 2008-10
Issue Date: 2016-11-14T08:06:52Z
Publisher: J. Fish. Soc. Taiwan
Abstract: ABSTRACT: Tetrodotoxin (TIX) is widely known marine neurotoxin, which acts by blocking the entry of sodium
ion into sodium channels of neuronal membranes . Toxicity was determined in two gastropods (Strombus
labiatus and Charonia tritonis) from Cebu Island, Philippines on October 2005, showing lethal potency in
the digestive gland of gastropod by using TIX bioassay. The average toxicity of gastropod specimens
was 28 ± 19 (mean ± S.D.) and 52 ± 17 MU/specimen in S. labiatusand C. tritonis, respectively. All toxic
extracts of each species samples were purified by passing through C18 Sep-Pack solid phase extraction
column and filtered through a microcentrifuge filter. The eluate was filtered through a microcentrifuge
filter. The filtrate was freeze-dried, dissolved in distilled water, and determined by high performance liquid
chromatography (HPLC) and liquid chromatograph tandem mass spectrometry (LC-MS/MS). The results
demonstrated that the toxin contained TIX. The paralytic shellfish poison (PSP) was not detected. The
evidence of TIX in both gastropods wams of potential problems in the Philippines gastropods.
Relation: 35(4)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38906
Appears in Collections:[食品科學系] 期刊論文

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