National Taiwan Ocean University Institutional Repository:Item 987654321/38792
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 28595/40626
造访人次 : 4206406      在线人数 : 59
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 进阶搜寻

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38792

题名: Species identification of snapper: A food poisoning incident in Taiwan.
作者: Wen-Feng Lin
Yao-Cheng Lyu
Ya-Jung Wu
Chi-Huan Lu
Deng-Fwu Hwang
贡献者: 國立臺灣海洋大學:食品科學系
关键词: PCR-RFLP
Species identification
Snapper
SDS-PAGE
日期: 2012-06
上传时间: 2016-11-09T03:34:36Z
出版者: Food Control
摘要: Abstract: A snapper (Lutjanidae fish) is a carnivorous coral reef fish that is distributed in sea areas around Taiwan. In December 2008 in southern Taiwan, a food poisoning incident occurred due to the ingestion of snapper, and the causative residue of ciguatera was investigated using a toxicity assay. To identify the species of the causative sample, six suspected species of Lutjanidae fish commonly found in Taiwan were analyzed using both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. According to the low molecular weight region (<30.0 kD) of species-specific patterns extracted from myofibrillar and sarcoplasmic proteins, the 6 snapper species could be clearly differentiated by the SDS-PAGE method. Furthermore, a consistent 465 bp sequence of the mitochondrial cytochrome b gene from the 6 snapper species was amplified by the PCR method and was rapidly distinguished by the analysis of restriction enzymes. According to both SDS-PAGE and PCR-RFLP methods, the poisonous sample was identified as Lutjanus bohar, which is also a notorious Lutjanidae species containing ciguateric toxins.
關聯: 25(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38792
显示于类别:[食品科學系] 期刊論文

文件中的档案:

档案 描述 大小格式浏览次数
index.html0KbHTML62检视/开启


在NTOUR中所有的数据项都受到原著作权保护.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈