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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38792

Title: Species identification of snapper: A food poisoning incident in Taiwan.
Authors: Wen-Feng Lin
Yao-Cheng Lyu
Ya-Jung Wu
Chi-Huan Lu
Deng-Fwu Hwang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: PCR-RFLP
Species identification
Snapper
SDS-PAGE
Date: 2012-06
Issue Date: 2016-11-09T03:34:36Z
Publisher: Food Control
Abstract: Abstract: A snapper (Lutjanidae fish) is a carnivorous coral reef fish that is distributed in sea areas around Taiwan. In December 2008 in southern Taiwan, a food poisoning incident occurred due to the ingestion of snapper, and the causative residue of ciguatera was investigated using a toxicity assay. To identify the species of the causative sample, six suspected species of Lutjanidae fish commonly found in Taiwan were analyzed using both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. According to the low molecular weight region (<30.0 kD) of species-specific patterns extracted from myofibrillar and sarcoplasmic proteins, the 6 snapper species could be clearly differentiated by the SDS-PAGE method. Furthermore, a consistent 465 bp sequence of the mitochondrial cytochrome b gene from the 6 snapper species was amplified by the PCR method and was rapidly distinguished by the analysis of restriction enzymes. According to both SDS-PAGE and PCR-RFLP methods, the poisonous sample was identified as Lutjanus bohar, which is also a notorious Lutjanidae species containing ciguateric toxins.
Relation: 25(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38792
Appears in Collections:[食品科學系] 期刊論文

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