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Title: Hemolytic activity of venom from crown-of-thorns starfish Acanthaster plancispines
Authors: Chi-Chiu Lee
Wann-Sheng Tsai
Hernyi Justin Hsieh
Deng-Fwu Hwang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Hemolysis
Crown-of-thorns starfish
Date: 2013-09
Issue Date: 2016-11-09T03:19:00Z
Publisher: Journal of Venomous Animals and Toxins including Tropical Diseases
Abstract: Abstract: The crown-of-thorns starfish Acanthaster planci is a venomous species from Taiwan whose venom provokes strong hemolytic activity. To understand the hemolytic properties of A. planci venom, samples were collected from A. planci spines in the Penghu Islands, dialyzed with distilled water, and lyophilized into A. planci spine venom (ASV) powder. Both crude venom and ASV cause 50% hemolysis at a concentration of 20 mug/mL. The highest hemolytic activity of ASV was measured at pH 7.0-7.4; ASV-dependent hemolysis was sharply reduced when the pH was lower than 3 or greater than 8. There was almost no hemolytic activity when the Cu2+ concentration was increased to 10 mM. Furthermore, incubation at 100[degree sign]C for 30 to 60 minutes sharply decreased the hemolytic activity of ASV. After treatment with the protease alpha-chymotrypsin, the glycoside hydrolase cellulase, and the membrane component cholesterin, the hemolytic activity of ASV was significantly inhibited. The results of this study provide fundamental information about A. planci spine venom. The hemolytic activity was affected by pH, temperature, metal ions, EDTA, cholesterin, proteases, and glycoside hydrolases. ASV hemolysis was inhibited by Cu2+, cholesterin, alpha-chymotrypsin, and cellulose, factors that might prevent the hemolytic activity of venom and provide the medical treatment for sting.
Relation: 19(1):22
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38789
Appears in Collections:[食品科學系] 期刊論文

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