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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38785

Title: Cytotoxicity of venom from crown-of-thorns starfish (Acanthaster planci) spine
Authors: Chi-Chiu Lee
Wann-Sheng Tsai
Hernyi-Justin Hsieh
Deng-Fwu Hwang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Cytotoxicity
Crown-of-thorns starfish
Acanthaster planci
Spine
Venom
Date: 2013-06
Issue Date: 2016-11-09T02:45:37Z
Publisher: Molecular & Cellular Toxicology
Abstract: Abstract: The cytotoxic effects of the crown-ofthorns starfish Acanthaster planci spine venom (ASV) in five cell lines, including human neuroblastoma (SHSY5Y), human hepatocellular carcinoma (HepG2), human melanoma (A375.S2), human skin fibroblast (CCD-966SK) and mouse macrophage-like cell (RAW 264.7) were assayed. The results indicated that ASV showed cytotoxic effects depending on dose in these five cell lines. Specifically, ASV significantly inhibited the proliferation of human melanoma cell line A375.S2 at 10 μg/mL, indicating A. planci spine venom could be utilized as potential chemotherapeutic agent in the treatment of cancer. The cytotoxicity of ASV to human melanoma cell line A375.S2 was relatively well retained at temperature less than 40°C, and sharply lost at temperature more than 80°C. The cytotoxicity of ASV also sharply lost at extreme pH environments (pH 2 and 12). The cytotoxicity of ASV was attenuated when treated with Cu2+ and anti-oxidant N-acetylcysteine. After SDS-PAGE analysis, ASV showed the major protein components ranging from 10 kDa to 37 kDa.
Relation: 9(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38785
Appears in Collections:[食品科學系] 期刊論文

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