National Taiwan Ocean University Institutional Repository:Item 987654321/38516
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题名: Protein A-conjugated luminescent gold nanodots as a label-free assay for immunoglobulin G in plasma
作者: Yen-Chun Shiang;Che-An Lin;Chih-Ching Huang;Huan-Tsung Chang
贡献者: 國立臺灣海洋大學:生命科學暨生物科技學系
日期: 2011
上传时间: 2016-09-08T05:54:49Z
出版者: Analyst
摘要: Abstract: We have employed protein A-modified gold nanodots (PA–Au NDs) as a luminescence sensor for the detection of human immunoglobulin G (hIgG) in homogeneous solutions. The luminescent PA–Au NDs were prepared simply by mixing protein A with the luminescent Au NDs (average diameter: ca. 1.8 nm). The specific interactions that occur between protein A and hIgG allowed us to use the PA–Au NDs to detect hIgG selectively. Under optimal conditions [10 nM PA–Au NDs (two protein A molecules per Au ND), 5.0 mM phosphate buffer solution, pH 7.4], the PA–Au ND probe detected hIgG with high sensitivity (limit of detection = 10 nM) and remarkable selectivity (>50-fold) over other proteins. In an assay that took advantage of the competition between protein G and the PA–Au NDs for IgG, we detected protein G at concentrations as low as 85 nM. This PA–Au ND probe allowed determination of the hIgG concentration in plasma samples without any need for sample pretreatment. Our results exhibited a good linear correlation (R2 = 0.97) with those obtained using an enzyme-linked immunosorbent assay. Our simple, sensitive, and selective approach appears to hold practical potential for use in the clinical diagnosis of immune diseases associated with changes in hIgG levels.
關聯: 6
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38516
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