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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38471

Title: Multivalent Aptamer/Gold Nanoparticle–Modified Graphene Oxide for Mass Spectrometry–Based Tumor Tissue Imaging
Authors: Rong-Cing Huang;Wei-Jane Chiu;Irving Po-Jung Lai;Chih-Ching Huang
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 2015
Issue Date: 2016-09-07T03:15:21Z
Publisher: Scientific Report
Abstract: Abstract
The protein mucin1 (MUC1) is an attractive target for cancer biomarkers because it is overexpressed in most adenocarcinomas. In this study, we exploited a MUC1-binding aptamer (AptMUC1) as a targeting agent for nanoparticle-based imaging systems coupled with laser desorption/ionization mass spectrometry (LDI-MS). We found that AptMUC1-conjugated gold nanoparticles immobilized, through hydrophobic and π–π interactions, on graphene oxide (AptMUC1–Au NPs/GO) bound effectively to MUC1 units on tumor cell membranes. The ultrahigh density and high flexibility of AptMUC1 on the GO surface enhanced the platform’s cooperative and multivalent binding affinity for MUC1 on cell membranes. After we had labeled MUC1-overexpressing MCF-7 cells (human breast adenocarcinoma cell line) with AptMUC1–Au NPs/GO, we used LDI-MS to monitor Au cluster ions ([Aun]+; n = 1–3), resulting in the detection of as few as 100 MCF-7 cells. We also employed this AptMUC1–Au NPs/GO–LDI-MS system to analyze four different MUC1 expression cell lines. In addition, the AptMUC1–Au NPs/GO platform could be used further as a labeling agent for tumor tissue imaging when coupled with LDI-MS. Thus, Apt–Au NPs/GO can function as a highly amplified signal transducer through the formation of large Au clusters ions during LDI-MS analysis.
Relation: 5
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38471
Appears in Collections:[生命科學系] 期刊論文

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