English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27320/39164
Visitors : 2475538      Online Users : 32
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38469

Title: Photoluminescent graphene quantum dots for in vivo imaging of apoptotic cells
Authors: Prathik Roy;Arun Prakash Periasamy;Chiu-Ya Lin;Guor-Mour Her;Wei-Jane Chiu;Chi-Lin Li;Chia-Lun Shu;Chih-Ching Huang;Chi-Te Liang;Huan-Tsung Chang
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 2015
Issue Date: 2016-09-07T02:58:01Z
Publisher: Nanoscale
Abstract: Abstract: Apoptosis (programmed cell death) is linked to many incurable neurodegenerative, cardiovascular and cancer causing diseases. Numerous methods have been developed for imaging apoptotic cells in vitro; however, there are few methods available for imaging apoptotic cells in live animals (in vivo). Here we report a novel method utilizing the unique photoluminescence properties of plant leaf-derived graphene quantum dots (GQDs) modified with annexin V antibody (AbA5) to form (AbA5)-modified GQDs (AbA5-GQDs) enabling us to label apoptotic cells in live zebrafish (Danio rerio). The key is that zebrafish shows bright red photoluminescence in the presence of apoptotic cells. The toxicity of the GQDs has also been investigated with the GQDs exhibiting high biocompatibility as they were excreted from the zebrafish's body without affecting its growth significantly at a concentration lower than 2 mg mL−1 over a period of 4 to 72 hour post fertilization. The GQDs have further been used to image human breast adenocarcinoma cell line (MCF-7 cells), human cervical cancer cell line (HeLa cells), and normal human mammary epithelial cell line (MCF-10A). These results are indispensable to further the advance of graphene-based nanomaterials for biomedical applications.
Relation: 6
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38469
Appears in Collections:[生命科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML91View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback