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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38468

Title: One-step synthesis of biofunctional carbon quantum dots for bacterial labeling
Authors: Cheng-I Weng
Huan-Tsung Chang
Chia-Hua Lin
Yu-Wei Shen
Binesh Unnikrishnan
Yu-Jia Li
Chih-Ching Huang
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Keywords: Carbon quantum dots
E. coli
Lectin
Bacterial labeling
Mannose
Date: 2015
Issue Date: 2016-09-07T02:25:35Z
Publisher: Biosensor and Bioelectronics
Abstract: Abstract: In this study, we used a simple one-step dry heating method to synthesize mannose-modified fluorescent carbon quantum dots (Man-CQDs) from solid ammonium citrate and mannose, and successfully applied for labeling Escherichia coli. The highly soluble Man-CQDs had an average particle diameter of 3.1±1.2 nm and exhibited a quantum yield of 9.8% at excitation and emission wavelengths of 365 and 450 nm, respectively. The fluorescent Man-CQDs could selectively bind to the FimH lectin unit in the flagella of the wild-type 1 E. coli K12 strain. We optimized the labeling efficiency of the Man-CQDs by controlling the ratio of ammonium citrate to mannose during their synthesis. The specific binding of the mannose units to E. coli allowed quantitative detection of the bacteria at levels down to 450 colony forming units mL−1 in lab samples, and facilitate the application of the Man-CQDs for bacterial analyses of real samples (tap water, apple juice, human urine). The synthesis of our Man-CQDs, their labeling, and their use in the detection of bacteria were all simple, inexpensive and efficient processes.
Relation: 68(15)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38468
Appears in Collections:[生命科學系] 期刊論文

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