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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38464

Title: Identification of Microalgae by Laser Desorption/Ionization Mass Spectrometry Coupled with Multiple Nanomatrices
Authors: Lung-Hsiang Peng
Binesh Unnikrishnan
Chi-Yu Shih
Tung-Ming Hsiung
Jeng Chang
Pang-Hung Hsu
Tai-Chia Chiu
Chih-Ching Huang
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Keywords: Nanostructures
Microalgae
Identification
Laser desorption/ionization mass spectrometry
Matrices
Date: 2016
Issue Date: 2016-09-07T01:37:22Z
Publisher: Marine Biotechnology
Abstract: Abstract: In this study, we demonstrate a simple method to identify microalgae by surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using three different substrates: HgSe, HgTe, and HgTeSe nanostructures. The fragmentation/ionization processes of complex molecules in algae varied according to the heat absorption and transfer efficiency of the nanostructured matrices (NMs). Therefore, the mass spectra obtained for microalgae showed different patterns of m/z values for different NMs. The spectra contained both significant and nonsignificant peaks. Constructing a Venn diagram with the significant peaks obtained for algae when using HgSe, HgTe, and HgTeSe NMs in m/z ratio range 100–1000, a unique relationship among the three sets of values was obtained. This unique relationship of sets is different for each species of microalgae. Therefore, by observing the particular relationship of sets, we successfully identified different algae such as Isochrysis galbana, Emiliania huxleyi, Thalassiosira weissflogii, Nannochloris sp., Skeletonema cf. costatum, and Tetraselmis chui. This simple and cost-effective SALDI-MS analysis method coupled with multi-nanomaterials as substrates may be extended to identify other microalgae and microorganisms in real samples.
Relation: 18(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38464
Appears in Collections:[生命科學系] 期刊論文

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