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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38461

Title: Self-Templated Formation of Aptamer-Functionalized Copper Oxide Nanorods with Intrinsic Peroxidase Catalytic Activity for Protein and Tumor Cell Detection.
Authors: Chien-wei Wu;Scott G. Harroun;Chia-Wen Lien;Chih-Ching Huang
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 2016
Issue Date: 2016-09-07T01:18:04Z
Publisher: Sensors and Actuators B Chemical
Abstract: Abstract: We have synthesized aptamer-functionalized copper nanoparticles (Apt–Cu NPs) using simple ascorbate-mediated reduction of Cu(II) ions in Apt-Tn templates comprising an aptamer (Apt) sequence with a poly(dT) motif at both the 3′- and 5′-termini. The as-prepared Apt–Cu NPs underwent rapid oxidation to Apt-Tn–CuO/Cu2O NPs. The CuO/Cu2O NPs tended to have rod-like shapes when the Apt-Tn templates had long poly(dT) units (n > 30). Interestingly, the as-formed Apt-Tn–CuO/Cu2O NPs exhibited intrinsic peroxidase-like activity for the H2O2-mediated oxidation of Amplex Red (AR) to fluorescent resorufin. The catalytic activity of thrombin binding aptamer-templated CuO/Cu2O NPs (TBA-Tn–CuO/Cu2O NPs) was significantly suppressed after the specific binding of thrombin to the aptamer units on the particles’ surfaces. The H2O2/AR–TBA-Tn–CuO/Cu2O NP probe provided a limit of detection (LOD) of thrombin in serum samples of 0.5 nM. Using Mucin1 binding aptamer-templated CuO/Cu2O NPs (AptMUC1-T30–CuO/Cu2O NPs), we applied this sensing platform to the detection of Mucin1-overexpressing tumor cells (LOD: ca. 100 cells). This study suggests that the Apt−Tn can be used as a universal template for preparation of enzyme-like CuO/Cu2O NPs for detection of proteins and labeling of tumor cells when using appropriate aptamers.
Relation: 227
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38461
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