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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38459

Title: Immobilization of aptamer-modified gold nanoparticles on BiOCl nanosheets: Tunable peroxidase-like activity by protein recognition.
Authors: Chia-Lun Hsu
Chia-Wen Lien
Chia-Wei Wang
Scott G. Harroun
Chih-Ching Huang
Huan-Tsung Chang
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Keywords: Synergistic effects
Enzyme
Self-assembly
Nanoparticles
Aptamers
Date: 2016
Issue Date: 2016-09-06T08:31:44Z
Publisher: Biosensors and Bioelectronics
Abstract: Abstract: A self-assembled nanocomposite is prepared from an aqueous mixture of aptamer-modified gold nanoparticles (Apt–Au NPs), bismuth ions and chloride ions. The Apt–Au NPs are immobilized on bismuth oxychloride (BiOCl) nanosheets in situ to form Apt–Au NPs/BiOCl nanocomposites. The as-prepared nanocomposites exhibit high peroxidase-like activity for the catalytic conversion of Amplex Red (AR) to fluorescent resorufin in the presence of H2O2. The catalytic activity of Apt–Au NPs/BiOCl nanocomposites is at least 90-fold higher than that of Apt–Au NPs or BiOCl nanosheets, revealing synergistic effects on their activity. The catalytic activity of Apt–Au NPs/BiOCl nanocomposites is suppressed by vascular endothelial growth factor-A165 (VEGF-A165) molecules that specifically interact with the aptamer units (Del-5-1 and v7t-1) on the nanocomposite surface. The AR/H2O2–Apt–Au NPs/BiOCl nanocomposites probe shows high selectivity (>1000-fold over other proteins) and sensitivity (detection limit ~0.5 nM) for the detection of VEGF-A165. Furthermore, the probe is employed for the detection of VEGF isoforms and for the study of interactions between VEGF and VEGF receptors. The practicality of this simple, rapid, cost-effective probe is validated by the analysis of VEGF-A165 in cell culture media, showing its great potential for the analysis of VEGF in biological samples.
Relation: 75
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38459
Appears in Collections:[生命科學系] 期刊論文

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