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http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38458
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| Title: | Batch production of high-content fructo-oligosaccharides from sucrose by the mixed-enzyme system of β-fructofuranosidase and glucose oxidase |
| Authors: | Jong Won Yun;Min Gyu Lee;Seung Koo Song |
| Contributors: | 國立臺灣海洋大學：生命科學暨生物科技學系 |
| Date: | 1994
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| Issue Date: | 2016-09-06T07:25:46Z
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| Publisher: | Journal of Fermentation and Bioengineering |
| Abstract: | Abstract: The production of high-content fructo-oligosaccharides from sucrose by the mixed-enzyme system of β-fructofuranosidase and glucose oxidase was investigated. The mixed-enzyme reaction was carried out in a stirred tank reactor containing 0.7 l of sucrose solution with coupled β-fructofuranosidase and glucose oxidase for 25 h. The optimum conditions for the mixed-enzyme reaction were as follows: pH, 5.5; temperature, 40°C; sucrose concentration, 400 g/l; agitation speed, 550 rpm; oxygen flow rate, 0.7 l/min; enzyme dosage, 10 units of β-fructofuranosidase with the combination of 15 units of glucose oxidase per gram sucrose. Under optimum conditions, high-content fructo-oligosaccharides up to 98% were obtained with complete consumption of sucrose and glucose by the mixed-enzyme system. Compared with the fructo-oligosaccharides produced by the β-fructofuranosidase, the high-content fructo-oligosaccharides produced by the mixed-enzyme system showed a significant difference with respect to sugar composition; i.e., a higher content of nystose was accumulated and only a trace amount of fructofuranosyl nystose was detected. |
| Relation: | 77(2) |
| URI: | http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38458 |
| Appears in Collections: | [生命科學系] 期刊論文
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