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Title: Characterization of Fish Cu/Zn–Superoxide Dismutase and Its Protection from Oxidative Stress
Authors: Chuian-Fu Ken
Chi-Tsai Lin
Jei-Fu Shaw
Jen-Leih Wu
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Keywords: Escherichia coli
Cu/Zn superoxide dismutase
Date: 2003
Issue Date: 2016-09-06T06:58:00Z
Publisher: Marine Biotechnology
Abstract: Abstract: Copper/zinc superoxide dismutase was cloned from the zebrafish (Danio rerio). The full coding region of the zebrafish superoxide dismutase (ZSOD) complementary DNA was ligated with pET-20b(+) and successfully expressed in Escherichia coli strain AD494(DE3)pLysS. The active enzyme was purified by His tagging. The ZSOD yield was 6 mg from 0.2 L of E. coli culture, and the specific activity was 2000 U/mg as assayed using a RANSOD kit. The enzyme stability was characterized by reaction to temperature, pH, and detergent treatment. The results showed enzyme activity was still active after heat treatment at 70°C for 10 minutes, resistant to pH treatment from 2.3 to 12, and resistant to treatment with sodium dodecyl sulfate (SDS) under 4%. In addition, the recombinant ZSOD was used to protect fish from 100 ppm of paraquat-induced oxidative injury by soaking fish larva in 55 µg/ml SOD enzyme. The results were significant.
Relation: 5(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38454
Appears in Collections:[生命科學系] 期刊論文

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