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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38447

Title: High-Level Production of Recombinant Chicken Cystatin by Pichia pastoris and Its Application in Mackerel Surimi
Authors: Gen-Hung Chen
Shye-Jye Tang
Ching-San Chen
Shann-Tzong Jiang
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Keywords: cysteine proteinase inhibitor
mackerel surimi
Recombinant cystatin
Pichia pastoris
Date: 2001
Issue Date: 2016-09-06T01:24:01Z
Publisher: Journal of Agriculture Food Chemical
Abstract: Abstract: A high level of the secreted form of recombinant chicken cystatin was expressed in Pichia pastoris X-33 by chromosomal integration of multiple copies of an expression cassette containing chicken cystatin under the control of glyceraldehyde-3-phosphate dehydrogenase promoter. The inhibition ability of the recombinant for papain-like proteinase was found to correspond to those of natural chicken cystatin. The recombinant cystatin substantially inhibited the proteolysis of myosin and gel softening, which consequently improved the gel properties of mackerel surimi.
Relation: 49(2)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38447
Appears in Collections:[生命科學系] 期刊論文

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