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Title: Acidic-Rich Region of Amyloid Precursor Protein Induces Glial Cell Apoptosis. Apoptosis
Authors: Sun KH;Sun GH;Su Y;Chang CI;Chuang MJ;Wu WL;Chu CY;Tang SJ.
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 2004
Issue Date: 2016-09-01T08:25:02Z
Publisher: Apoptosisi
Abstract: Abstract: Amyloid precursor protein (APP) has several caspase cleavage sites in its C-terminal cytoplasmic domain and N-terminal extracellular domain. Caspase cleavages of APP at its cytosolic tail may result in releasing the domain and inducing cell death. During apoptosis, the N-terminal domain may also be processed at amino acids 197 and 219 by caspases leading to unmasking of an acidic-rich region (AR). In this study, AR-exposing APP was shown to inhibit cell growth after transfection into RBA-1 astrocytes and BV-2 microglial cells. The recombinant AR from residue 220 to 288 of APP (APP220-288) was produced and its biological activities were analyzed. APP220-288 induced morphological changes, cell death, and DNA fragmentation in BV-2 and RBA-1 cells. However, AR was determined to have no apparent effects in suspension cells, erythroleukemia K562 cells, and Jurkat T cells. The cytotoxicity was depending on negative charge cluster and the apoptotic activity of AR was attributed to the inhibition of cell adhesion. In BV-2 microglial cells, AR significantly stimulated Fas expression, although expressions of the pro-inflammatory cytokine genes were not detected. APP220-288 also induced nitric oxide synthase (iNOS) expression and nitric oxide (NO) production. These findings indicate that the acidic-rich domain of APP may have apoptotic activity due to inhibition of cell adhesion and induction of iNOS and Fas expressions. Moreover, unmasking the apoptosis-induced AR may activate and exacerbate glial cells which in turn lead to further progression of the death program.
Relation: 9(6)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38444
Appears in Collections:[生命科學系] 期刊論文

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