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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38418

Title: Structure and transcription of genes within the β-hbd-adh1 region of Clostridium acetobutylicum P262
Authors: Jonathan S. Youngleson
Fu-Pang Lin
Sharon J. Reid
David R. Woods
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Keywords: Metronidazole
Clostridium acetobutylicum
Gene transcription
Solvent production
Date: 1995
Issue Date: 2016-09-01T02:59:40Z
Publisher: FEMS Microbiology Letters
Abstract: Abstract: The 1.2-kb DNA fragment upstream of the linked β-hbd (3-hydroxybutyryl-CoA dehydrogenase) and adh1 (NADPH-dependent alcohol dehydrogenase) genes from Clostridium acetobutylicum P262 was sequenced. The upstream region contained an open reading frame (ORFB) which was found to have 44% amino acid identity to the fixB gene products of yRhizobium and Azorhizobium. The β-hbd and ORFB genes were expressed during the acidogenic and solventogenic phases. The β-hbd gene was transcribed on a single mRNA species of 2.0 kb, whereas the ORFB gene was transcribed on two species of mRNA of 2.0 and 3.5 kb, respectively. The adh1 gene was induced or derepressed at the pH breakpoint before the onset of solventogenesis and was transcribed on a single species of mRNA of 2.4 kb.
Relation: 125(2-3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38418
Appears in Collections:[生命科學系] 期刊論文

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