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Title: Cloning, sequencing, expression and characterization of the manganese superoxide dismutase gene from Vibrio alginolyticus.
Authors: Shyu YC;Chiu CC;Lin FP.
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 1999
Issue Date: 2016-09-01T01:28:33Z
Publisher: Biochem Mol Biol Int.
Abstract: Abstract: The sodA gene coding for manganese superoxide dismutase from the marine microorganism Vibrio alginolyticus was cloned, sequenced and over-expressed in Escherichia coli using the pET20b (+) expression vector. The full-length gene was consisted of 603bp open reading frame, which encoded a polypeptide of 201 amino acid residues, with a calculated molecular weight of 22672Da. The deduced amino acid sequence of the sodA showed considerable homology to other Mn-SODs. The recombinant enzyme was efficiently purified from crude E. coli cell lysate by the metal ion affinity chromatography. The recombinant VAMn-SOD resisted thermo-denaturation up to 60 degrees C and was insensitive to inhibitors such as H2O2, NaN3 and diethyldithiocarbamic acid.
Relation: 47(5)
Appears in Collections:[Department of Life Science] Periodical Articles

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