English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27248/39091
Visitors : 2417188      Online Users : 62
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38415

Title: Cloning, sequencing, expression and characterization of the manganese superoxide dismutase gene from Vibrio alginolyticus.
Authors: Shyu YC;Chiu CC;Lin FP.
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 1999
Issue Date: 2016-09-01T01:28:33Z
Publisher: Biochem Mol Biol Int.
Abstract: Abstract: The sodA gene coding for manganese superoxide dismutase from the marine microorganism Vibrio alginolyticus was cloned, sequenced and over-expressed in Escherichia coli using the pET20b (+) expression vector. The full-length gene was consisted of 603bp open reading frame, which encoded a polypeptide of 201 amino acid residues, with a calculated molecular weight of 22672Da. The deduced amino acid sequence of the sodA showed considerable homology to other Mn-SODs. The recombinant enzyme was efficiently purified from crude E. coli cell lysate by the metal ion affinity chromatography. The recombinant VAMn-SOD resisted thermo-denaturation up to 60 degrees C and was insensitive to inhibitors such as H2O2, NaN3 and diethyldithiocarbamic acid.
Relation: 47(5)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38415
Appears in Collections:[生命科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML40View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback