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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38414

Title: Cloning and characterization of manganese superoxide dismutase gene from Vibrio parahaemolyticus and application to preliminary identification of Vibrio strains
Authors: Yu-Chiau Shyu;Fu-Pang Lin
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 1999
Issue Date: 2016-08-31T08:39:23Z
Publisher: IUBMB Life
Abstract: Abstract: The sodA gene coding for manganese superoxide dismutase (Mn-SOD) from the marine microorganism Vibrio parahaemolyticus was cloned, sequenced, and overexpressed in Escherichia coli by use of the pET20b (+) expression vector. The full-length gene consisted of a 588-bp open reading frame and encoded a polypeptide of 196 amino acid residues, with a calculated molecular mass of 21 713 Da. The recombinant enzyme was efficiently purified from crude E. coli cell lysate by metal ion affinity chromatography. The recombinant VPMn-SOD resisted thermo-denaturation up to 60 °C and was insensitive to such inhibitors as EDTA, NaN3 and diethyldithiocarbamic acid. The specificity of V. parahaemolyticus Mn-SOD gene probe was analyzed by cross-species polymerase chain reaction to provide information for Vibrio strain identification.
Relation: 48(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38414
Appears in Collections:[生命科學系] 期刊論文

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