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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38398

Title: Expression of Estrogen Receptors Alfa and Beta mRNA and Alkaline Phosphatase in the Differentiation of Osteoblasts from Elderly Postmenopausal Women: Comparison with Osteoblasts from Osteosarcoma Cell Lines
Authors: Fang-Ping Chen
Todd Hsu
Chin-Hwa Hu
Wen-Der Wang
Kun-Chuang Wang
Li-Fen Teng
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Keywords: alkaline phosphatase
estrogen receptor a mRNA
estrogen receptor b mRNA
human osteosarcoma cell lines
postmenopausal women
primary human osteoblasts
Date: 2006
Issue Date: 2016-08-31T06:28:33Z
Publisher: Taiwanese Journal of Obstetrics and Gynecology
Abstract: Abstract:
To evaluate the expression of estrogen receptors (ER) a and b, and activity of alkaline phosphatase during differentiation of primary osteoblast cells (hOB) from aged postmenopausal women and human osteosarcoma cell lines (HOS, MG63).
Materials and Methods
Osteoblast cultures were prepared from the upper femur of postmenopausal patients (age, 60-74 years) and HOS. At the indicated times (days 5, 10, 15, 20, and 25), alkaline phosphatase activity and expression of ERa and ERb mRNA were evaluated.
In both cultures of primary hOB and HOS, alkaline phosphatase activity decreased at the osteoblast proliferation stage, whereas it subsequently increased at the matrix maturation stage. ERb mRNA was strongly expressed in HOS on day 15 and remained at high levels of transcription through to day 25 (matrix maturation phase), whereas ERa mRNA was barely detectable during osteoblast differentiation. In hOB, transcription of ERa mRNA was much stronger than that of ERb mRNA.
The presence of ERa and ERb mRNA in osteoblasts supports the involvement of estrogen in human bone formation. The developmental expression of alkaline phosphatase was not correlated to ER mRNA expression during osteoblast differentiation. ER isoforms may have different functions or interact with each other during osteoblast differentiation. Since the expression of ER isoforms is different between postmenopausal women and osteosarcoma cell lines, characteristics of osteosarcoma cell lines may not be suitable as a model for the evaluation of estrogen effects on postmenopausal osteoporosis.
Relation: 45(4)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38398
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