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Abstract: An immuno-probe against a glycoprotein in the egg chorion was developed for egg identification.
The 97 kD glycoprotein in the chorion of unfertilized eggs of Epinephelus malabaricus was
isolated and separated by SDS-PAGE as an antigen to induce antibody from rabbit. The
reactivity of the antibody as the immuno-probe to E. malabaricus eggs was significantly
positive, and was specific in that it did not react with the eggs of other fish species. The
immuno-probe should be useful in identifying the eggs of E. malabaricus among mixed egg