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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38386

Title: Mutual adaptation between mouse transglutaminase 4 and its ative substrates in the formation of copulatory plug
Authors: Huan-Chin Tseng;Jyh-Bing Tang;P S Sudhakar Gandhi;Yee-Hsiung Chen;Ching-Wei Luo;Chung-Mao Ou;Chia-Jen Tseng;Han-Jia Lin
Contributors: 國立臺灣海洋大學:生命科學暨生物科技學系
Date: 2011
Issue Date: 2016-08-31T03:12:38Z
Publisher: Amino Acids
Abstract: Abstract: Formation of copulatory plugs by male animals is a common means of reducing competition with rival males. In mice, copulatory plugs are formed by the coagulation of seminal vesicle secretion (SVS), which is a very viscous and self-clotting fluid containing high concentration of proteins. In its native state, mouse SVS contains a variety of disulfide-linked high-molecular-weight complexes (HMWCs) composed of mouse SVS I-III, which are the major components of mouse SVS. Further, mouse SVS I-III are the substrates for transglutaminase 4 (TGM4), a cross-linking enzyme secreted from the anterior prostate. According to activity assays, mouse TGM4 prefers a mild reducing and alkaline environment. However, under these conditions, the activity of mouse TGM4 toward SVS I-III was much lower than that of a common tissue-type TGM, TGM2. On the other hand, mouse TGM4 exhibited much higher cross-linking activity than TGM2 when native HMWCs containing SVS I-III were used as substrates under non-reducing condition. By the action of TGM4, the clot of SVS became more resistant to proteolysis. This indicates that the activity of TGM4 can further rigidify the copulatory plug and extend its presence in the female reproductive tract. Together with the properties of TGM4 and the nature of its disulfide-linked SVS protein substrates, male mice can easily transform the semen into a rigid and durable copulatory plug, which is an important advantage in sperm competition.
Relation: 42(2-3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38386
Appears in Collections:[生命科學系] 期刊論文

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