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题名: Using photoluminescent gold nanodots to detect hemoglobin in diluted blood samples
作者: Li-Yi Chen
Chih-Ching Huang
Wei-Yu Chen
Han-Jia Lin
Huan-Tsung Chang
贡献者: 國立臺灣海洋大學:生命科學暨生物科技學系
关键词: Diluted blood samples
Hemin
Photoluminescent gold nanodots
Hemoglobin
日期: 2013
上传时间: 2016-08-31T02:59:02Z
出版者: Biosensors and Bioelectronics
摘要: Abstract: In this study we used photoluminescent 11-mercaptoundecanoic acid-bound gold nanodots (11-MUA-Au NDs) to detect hemoglobin through photoluminescence (PL) quenching. The mechanism of quenching, which occurred through redox reactions between the 11-MUA-Au NDs and the Fe(II) atoms of hemin units, was supported by an increase in the signals (G 2.0 and 5.9) of high-spin state Fe(III) ions. The Stern–Volmer quenching constants (Ksv) for hemin, cytochrome c, hemoglobin, and myoglobin were 5.6×107, 1.7×107, 1.6×107, and 6.2×106 M−1, respectively, in good agreement with the order of their reduction potentials. When excited at 375 nm, the PL intensity of the 11-MUA-Au NDs at 520 nm decreased upon increasing the concentration of hemoglobin from 1.0 to 10 nM (R2=0.9913). This approach using bovine serum albumin blocked 11-MUA-Au NDs provided a limit of detection for hemoglobin (at a signal-to-noise ratio of 3) of 0.5 nM in biological buffer, with great selectivity over other non-heme-containing proteins, including human serum albumin, β-casein, and carbonic anhydrase. We validated the practicality of this approach through the determination of the concentrations (1.85–2.46 mM) of hemoglobin in diluted (106-fold) human blood samples based on PL quenching of Au NDs. This simple, sensitive, and selective approach holds great potential for the diagnosis of several diseases, including anemia, erythrocytosis, and thalassemias.
關聯: 43(15)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38383
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