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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38378

Title: A potential methodology for differentiation of ciguatoxin-carrying species of moray eel
Authors: Wei-Lung Tsai;Hong-Ming Chen;Cheng-Hong Hsieh;Deng-Fwu Hwang
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2009
Issue Date: 2016-08-31T01:34:18Z
Publisher: Food Control
Abstract: Abstract: A food poisoning incident due to ingestion of an unknown moray eel occurred in Taiwan. To identify the species of causative moray eel implicated in food poisoning, a 376-bp fragment sequence of cytochrome b gene was successfully obtained from four species of moray eel by using a pair of primers (L newest-1/H 15149). This fragment could be amplified using fish meat treated with different heating processes (100 °C for 30 min, 100 °C for 60 min, 121 °C for 15 min, and 121 °C for 30 min). After sequencing, it was found that no variation in sequence was detected among individuals within each species. The species of causative moray eel implicated in food poisoning was judged as Gymnothorax javanicus based on sequence analysis. In addition, using restriction enzyme analysis, including Taq I and Sau96 I, could distinguish these four species of moray eel and identify the fish species of poisoning sample as G. javanicus. The toxicity of viscera in 24 specimens of four moray eel species was not detected, but the food poisoning sample was found to be toxic. Overall, this study proved that DNA sequence and restriction enzyme analyses are useful in identifying that the causative moray eel species was G. javanicus.
Relation: 20(6)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38378
Appears in Collections:[水產養殖學系] 期刊論文

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