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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38352

Title: Isolation and characterization of pathogenic Vibrio alginolyticus from diseased cobia Rachycentron canadum, cobia Rachycentron canadum
Authors: Ping-Chung Liu;Ji-Yang Lin;Pei-Tze Hsiao;Kuo-Kau Lee
Contributors: 國立臺灣海洋大學:水產養殖學系
Date: 2004
Issue Date: 2016-08-29T08:45:37Z
Publisher: Journal of Basic Microbiology
Abstract: Abstract: Outbreaks of serious mortality among cultured juvenile cobia Rachycentron canadum L. (weighing 8-10 g) characterized by lethargy, dark skin and ascites in the peritoneal cavity while some fish possessing damaged eyes occurred in July and August of 2001 in Taiwan. Fifteen motile bacterial strains were isolated from head kidney and/or the ascites on tryptic soy agar supplemented with 1% NaCl (TSA1) and/or thiosulphate citrate bile salt (TCBS) sucrose agar plates during the two outbreaks. All the isolates were characterized and identified as Vibrio alginolyticus on the basis of biochemical characteristics, and comparisons with those of the reference strain V. alginolyticus ATCC 17749. The strain C3c01 (a representative of the 15 similar field isolates), was virulent to the cobia with an LD50 value of 3.28 x 10(4) colony forming units/g fish body weight. All the moribund/dead fish exhibited lethargy, dark skin and ascites in the peritoneal cavity as that observed in natural outbreaks. The same bacteria could be reisolated from kidney and the ascites of fish after bacterial challenge using TSA1 and TCBS plates. The results reveal that V. alginolyticus is an infectious agent of vibriosis in the cobia.
Relation: 24(1)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38352
Appears in Collections:[水產養殖學系] 期刊論文

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