English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27329/39174
Visitors : 2479703      Online Users : 36
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38344

Title: Evaluation of damage in pacific oyster (Crassostrea gigas) spermatozoa before and after cryopreservation using comet assay
Authors: Gwo, Jin-Chywan
Wu, Chen-Yen
Chang,Wu-Shu Peter
Cheng, Hsien-Yu
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: comet assay
DNA
freeze
mollusks
sperm
oyster
Date: 2003
Issue Date: 2016-08-29T06:55:10Z
Publisher: Cryoletters
Abstract: Abstract: We examined the applicability of the comet assay (single cell gel electrophoresis assay) to estimate the quality of frozen-thawed Pacific oyster (Crassostrea gigas) spermatozoa. Comet assay was performed on semen before and after cryopreservation followed by fluorescent staining with propidium iodide to assess DNA integrity. After cryopreservation, the percentage of spermatozoa with damaged DNA significantly increased, while only about half of the cells displayed intact DNA, even when protected with 10% DMSO. All the considered parameters (head length, head area, head intensity, total length, total area, total intensity, tail length%, tail area%, and tail intensity%) were higher than the oyster sperm protected with 10%DMSO-artificial sea water after freezing and thawing. Only tail length%, tail area%, and tail intensity% were increased significantly after cryopreservation. The tail length % was found to be the most sensitive indicator of the cryopreservation-induced DNA damage. Our freeze-thawing procedure significantly affected oyster sperm DNA, as indicated by the reduced fertilization rate when frozen-thawed oyster sperm are used. Irreversible alteration of the genome may prevent fertilization or alter normal embryonic development. This study is the first to demonstrate that the comet assay is an inexpensive, rapid and sensitive method for determining DNA damage in Pacific oyster sperm quality assessments.
Relation: 24(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38344
Appears in Collections:[水產養殖學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML64View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback