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Title: Response to acute changes in salinity of two different muscle type creatine kinase isoforms, from euryhaline teleost (Oreochromis mossambicus) gills.
Authors: Hong-Yi Gong
Jen-Leih Wu
Wei-Tung Huang
Cliff Ji-Fan Lin
Ching-Feng Weng
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: Mozambique tilapia
Muscle type CK isoform
Creatine kinase
Date: 2004
Issue Date: 2016-08-25T08:03:58Z
Publisher: Biochimica et Biophysica Acta (BBA) - General Subjects
Abstract: Abstract: Two CKM isoforms (CKM1 and CKM2) from the gills of tilapia (Oreochromis mossambicus) were obtained after transfer from freshwater (FW) to seawater (SW, 25 ppt). Based on the 5′ and 3′ RACE, the identity of CKM1 and CKM2 was determined to be 59% in the 5′-untranslated region (5′-UTR) and 41.9% in the 3′-UTR. Using Northern blot hybridization with the CKM1 and CKM2 3′-UTR probes, CKM1 and CKM2 were found to be expressed in muscle, heart and gill. The levels of these two different CK isoforms (CKM1 and CKM2) were shown to be different in FW than after acute SW transfer, showing that CKM isoforms respond to changes in salinity.
Relation: 1675(1)
Appears in Collections:[Department of Aquaculture] Periodical Articles

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