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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38330

Title: Zebrafish anti-apoptotic protein zfBcl-xL can block betanodavirus protein alpha-induced mitochondria-mediated secondary necrosis cell death.
Authors: Horng-Cherng Wu
Chuan-Sheng Chiu
Jen-Leih Wu
Hong-Yi Gong
Ming-Chyuan Chen
Ming-Wei Lu
Jiann-Ruey Hong
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: Secondary Necrosis
Mitochondria
Mitochondrial Membrane Potential MMP
Cytochrome C
Cytosol
Date: 2008
Issue Date: 2016-08-25T07:36:11Z
Publisher: Fish & Shellfish Immunology
Abstract: Abstract: Betanodavirus protein a induces cell apoptosis or secondary necrosis by a poorly understood process. In the present work, red spotted grouper nervous necrosis virus (RGNNV) RNA 2 was cloned and transfected into tissue culture cells (GF-1) which then underwent apoptosis or post-apoptotic necrosis. In the early apoptotic stage, progressive phosphatidylser-ine externalization was evident at 24 h post-transfection (p.t.) by Annexin V-FLUOS staining. TUNEL assay revealed apoptotic cells at 24e72 h p.t, after which post-apoptotic necrotic cells were identified by acridine orange/ethidium bromide dual dye staining from 48 to 72 h p.t. Protein a induced progressive loss of mitochondrial membrane potential (MMP) which was detected in RNA2-transfected GF-1 cells at 24, 48, and 72 h p.t., which correlated with cyto-chrome c release, especially at 72 h p.t. To assess the effect of zfBcl-x L on cell death, RNA2-transfected cells were co-transfected with zfBcl-x L. Co-transfection of GF-1 cells prevented loss of MMP at 24 h and 48 h p.t. and blocked initiator caspase-8 and effector caspase-3 activation at 48 h p.t. We conclude that RGNNV protein a induces apoptosis followed by secondary necrotic cell death through a mitochondria-mediated death pathway and activation of caspases-8 and-3. Please cite this article in press as: Wu H-C et al., Zebrafish anti-apoptotic protein zfBcl-x L can block betanodavirus protein a-induced mitochondria-mediated secondary necrosis cell death.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/38330
Appears in Collections:[水產養殖學系] 期刊論文

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