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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37828

Title: Green turtle population structure in the Pacific: new insights from single nucleotide polymorphisms and microsatellites
Authors: Suzanne E. Roden;Phillip A. Morin;Amy Frey;George H. Balazs;Patricia Zarate;I-Jiunn Cheng;Peter H. Dutton
Contributors: 國立台灣海洋大學:海洋生物研究所
Keywords: Green turtle;Chelonia mydas;Single nucleotide polymorphism;SNP;Microsatellite;Sea turtle;Genetics;Conservation
Date: 2013
Issue Date: 2016-05-16T01:19:42Z
Abstract: ABSTRACT:A set of nuclear single nucleotide polymorphisms (SNPs) and microsatellite markers was used to detect genetic stock structure among 5 Pacific green turtle Chelonia mydas nesting populations. We sampled populations in the Galapagos Islands, Ecuador (n = 57), Colola, Mexico (n = 75), French Frigate Shoals, Hawaii (n = 141), Yap, Micronesia (n = 73), and Wan-an, Taiwan (n = 57), to represent eastern, central, and western Pacific regions. A combination of 29 single independent SNPs and linked SNPs combined as haplotypes were used for a total of 20 independent markers. In addition, 8 polymorphic microsatellite markers were applied to the same sample set. Both sets of nuclear markers confirmed significant differentiation between all sampled populations in the 3 Pacific regions (p ≤ 0.001). The use of these SNPs and microsatellites resulted in sufficient power to detect small population differences not seen in previous studies using smaller numbers of nuclear markers. Our results suggest that male-mediated gene flow between regional nesting stocks is more limited than previously believed, allowing the potential to delineate stocks more clearly. Finally, we discuss the value of SNP markers as an alternative or complement to other nuclear markers such as microsatellites for the examination of stock structure.
Relation: 20,pp.227-234
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37828
Appears in Collections:[海洋生物研究所] 期刊論文

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