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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37788

Title: Endocytic pathway is indicated for white spot syndrome virus (WSSV) entry in shrimp
Authors: Zih-Jhan Huang;Shih-Ting Kang;Jiann-Horng Leu;Li-Li Chen
Contributors: 國立台灣海洋大學:生命科學系
Keywords: White spot syndrome virus (WSSV);Primary cultured hemocytes;Endosome;Caveolae-mediated endocytosis
Date: 2013
Issue Date: 2016-05-09T08:45:37Z
Publisher: Fish & Shellfish Immunology
Abstract: Abstract:The white spot syndrome virus (WSSV) has had a serious economic impact on the global shrimp aquaculture industry in the past two decades. Although research has clarified a lot about its genome and structure, the mechanism of how WSSV enters a cell is still unclear. In this study to determine this mechanism, primary cultured hemocytes were used as an experimental model to observe the process of WSSV entry because the stable shrimp cell lines for WSSV infection are lacking. After labeling virions and endosomes with fluorescent dyes followed by observation with a confocal microscope, the results show that the WSSV colocalizes with early endosomes. Hemocytes are further treated with different endocytic inhibitors, methyl-β-cyclodextrin (MβCD) and chlorpromazine (CPZ). WSSV still can be detected in the hemocytes treated with CPZ, but not in the hemocytes treated with MβCD. Thus, we conclude that WSSV adopts the caveolae-mediated endocytosis to enter the shrimp cell.
Relation: 35(3),pp.707-715
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37788
Appears in Collections:[生命科學系] 期刊論文

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