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Title: Silver Nanoparticles as a Glucose Oxidase Immobilization Matrix for Amperometric Glucose Biosensor Construction
Authors: Fu-Yin Hsu;Ding‐Syuan Yu;Jung‐Che Chang;Chia‐Lin Chuang
Contributors: 國立台灣海洋大學:生命科學系
Keywords: Silver nanoparticles;Glucose oxidase;Immobilization;Biosensor
Date: 2011-10
Issue Date: 2016-05-09T07:52:17Z
Publisher: Journal of the Chinese Chemical Society
Abstract: ABSTRACT:Immobilized biomolecules are widely applied in medical, food and environmental fields. In this paper, glucose oxidase (GOD) was covalently immobilized on the surface of thiol-modified silver nanoparticles (AgNPs) to fabricate GOD-AgNP bioconjugate complexes. GOD-AgNP complex were more stable at high temperature and in high pH environments than the free GOD in solution. Additionally, the fabricated Carbon rod/GOD-AgNPs/Nafion/Chitosan electrode exhibited a rapid response and a linear calibration range from 0.5 mM to 6 mM for the detection of glucose. The Nafion/Chitosan coating could eliminate common interferents, such as uric acid and acetaminophen. As a conclusion, the experimental results indicated that the resulting modified electrode possesses an excellent response performance on glucose.
Relation: 58(6)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37780
Appears in Collections:[生命科學系] 期刊論文

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