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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37773

Title: Bone marrow mesenchymal stem cells, platelet-rich plasma and nanohydroxyapatite–type I collagen beads were integral parts of biomimetic bone substitutes for bone regeneration
Authors: Bo-Nian Lin;Shu Wen Whu;Chih-Hwa Chen;Fu-Yin Hsu;Jyh-Cheng Chen;Hsia-Wei Liu;Chien-Hao Chen;Hau-Min Liou
Contributors: 國立台灣海洋大學:生命科學系
Keywords: biomimetic;bone substitute;platelet-rich plasma;nanohydroxyapatite–type I collagen beads;bone marrow mesenchymal stem cell;bone regeneration
Date: 2012-06
Issue Date: 2016-05-09T02:51:36Z
Publisher: Journal of Tissue Engineering and Regenerative Medicine
Abstract: Abstract:Platelet rich plasma (PRP), which includes many growth factors, can activate osteoid production, collagen synthesis and cell proliferation. Nanohydroxyapatite-type I collagen beads (CIB), which mimetic natural bone components, are not only flexible fillers for bone defect but also encourage osteogenesis. Bone marrow mesenchymal stem cells (BMSCs) are often used as an abundant cell source for tissue engineering. We used a rabbit model to combine PRP, CIB and BMSCs (CIB+PRP+BMSC) into a bone-like substitute to study its impact on bone regeneration, when compared to defect alone, PRP, CIB+PRP, and PRP+BMSC. CIB+PRP upregulated more alkaline phosphatase (ALP) activity in BMSCs than PRP alone at 4 weeks postoperation. CIB+PRP+BMSC and PRP+BMSC did not differ significantly in DNA content, total collagen content, and ALP activity at 8 weeks. In histological assay, both CIB+PRP+BMSC and PRP+BMSC showed more bone regeneration at 4 and 8 weeks. Higher trabecular bone volume in tissue volume (BV/TV) (31.15±2.67% and 36.93±1.01%), fractal dimension (FD) (2.30±0.18 and 2.65±0.02) and lower trabecular separation (Tb.Sp) (2.30±0.18 and 1.35±0.16) of CIB+PRP+BMSC than of other groups at 4 and 8 weeks, and approach to of bone tissue (BV/TV=24.35±2.13%; FD=2.65±0.06; Tb.Sp=4.19±0.95). CIB+PRP+BMSC significantly enhanced new bone formation at 4 week. Therefore, nanohydroxyapatite-type I collagen beads combined with PRP and BMSCs produced a bone substitute with efficiently improved bone regeneration that shows promise to repair bone defects.
Relation: 7(11),pp.841–854
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37773
Appears in Collections:[生命科學系] 期刊論文

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