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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37768

Title: A biomimetic extracellular matrix composed of mesoporous bioactive glass as a bone graft material
Authors: Fu-Yin Hsu;Ru-Chun Weng;Hsiu-Mei Lin;Yi-Hsuan Lin;Meng-Ru Lu;Jing-Lun Yu;Hsien-Wen Hsu
Contributors: 國立台灣海洋大學:生命科學系
Keywords: Mesoporous bioactive glass;Nanofiber;Extracellular matrix;Drug carrier;Bone graft material
Date: 2015
Issue Date: 2016-05-09T01:14:22Z
Publisher: Microporous and Mesoporous Materials
Abstract: Abstract:Mesoporous bioactive glass (MBG) has been demonstrated to play an important role in bone regeneration. In this study, the fabrication of a matrix composed of MBG nanofibers (MBGNFs) that mimics the three-dimensional structure of the natural extracellular matrix is reported. The fabrication process utilized a sol–gel/electrospinning technique. The morphology, composition, and structure of the MBGNF matrices were characterized. The MBGNF matrices were typically characterized by highly ordered, one-dimensional channels in a hexagonally packed mesostructure. The drug loading and release profiles of the MBGNF matrices were also investigated. MBGNF matrices had better drug-loading efficiency and could reduce the burst release of gentamicin sulfate and prolong its release over 10 days. Hence, MBGNF matrices are suitable as a drug carrier. Additionally, immersing an MBGNF matrix in a simulated body fluid resulted in the formation of a layer of bone-like apatite minerals on the surface of the MBGNFs. This finding demonstrated the excellent in vitro bioactivity of the MBGNF matrix. Based on a cellular adhesion assay and an analysis of cytoskeletal organization, we determined that MBGNF matrices provided an appropriate environment for cellular adhesion. The observed cellular proliferation; alkaline phosphatase activity; and protein expression levels of osteopontin, osteocalcin and bone sialoprotein demonstrated that MBGNF matrices promoted the proliferation, differentiation and mineralization of MG63 osteoblast-like cells. Finally, the bone regeneration ability of the MBGNF matrix was evaluated using a rat calvarial defect model. The results revealed that MBGNF matrices were biodegradable and enhanced bone regeneration. Therefore, given the above results, the MBGNF matrix has the potential to become a new bone graft material for bone tissue engineering applications.
Relation: 212,pp.56-65
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37768
Appears in Collections:[生命科學系] 期刊論文

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