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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37666

Title: Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio)
Authors: Fu-Lung Yeh;Todd Hsu
Contributors: 國立台灣海洋大學:生命科學系
Date: 2002
Issue Date: 2016-04-11T01:23:18Z
Publisher: JOURNAL OF EXPERIMENTAL ZOOLOGY
Abstract: Abstract:A spontaneous high expression of heat shock protein 70 (HSP 70) was found to arise in zebrafish (Danio rerio) at the larval stage (84 hr after fertilization). The level of HSP 70 in 84-hr-old larvae was estimated to be six- to eightfold that of 12-hr-old embryos. As heat-induced HSP 70 synthesis in many eukaryotic organisms is known to be mediated by a transcriptional-dependent pathway activated by heat shock factor 1 (HSF-1), we then examined if the spontaneous and heat-induced HSP 70 synthesis in zebrafish were controlled by the same mechanism. Although the transient increase of a 62-kDa HSF-1-like polypeptide in 72- to 96-hr-old larvae seemed to correlate with the onset of the spontaneous HSP 70 production, an anti-HSF-1 antibody cocktail supershifted the heat shock element (HSE) binding complex induced by stressed but not by unstressed zebrafish extracts. Northern blot and quantitative RT-PCR analysis demonstrated the predominant presence of the cognate form of hsp 70 mRNA (hsc 70 mRNA) in developing zebrafish. The extent of heat-induced HSP 70 production in 84-hr-old larvae matched well with a dramatic increase in hsp 70 mRNA accumulation, while no apparent increase in total hsp 70 mRNA could be detected in 72- to 84-hr-old unstressed larvae by northern blot analysis. The stable expression of hsc 70 mRNA specific to beta-actin mRNA in normal zebrafish was confirmed by RT-PCR analysis. Hence, the spontaneous high expression of HSP 70 in zebrafish is believed to be controlled by a mechanism different from the HSF-1-dependent transcriptional activation of hsp 70 under heat stress.
Relation: 293(4),pp.349-59
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37666
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