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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37640

Title: https://www.researchgate.net/publication/222372399_Differential_effects_of_heavy_metals_on_the_binding_of_Xenopus_upstream_binding_factorxUBF_to_DNA
Authors: Todd Hsu;Hua-Met Huang;Chin-Hwa Hu
Contributors: 國立台灣海洋大學:生命科學系
Date: 1998-05
Issue Date: 2016-04-07T07:54:16Z
Abstract: Abstract:Xenopus upstream binding factor (xUBF) is a transcription factor for RNA polymerase I which contains multiple DNA-binding motifs. Among these DNA-binding motifs,HMG box I is essential for promoting RNA polymerase I-dependent rRNA gene transcription. Gel shift assay indicated that the binding of recombinant HMG box I to a 136-bp linear DNA probe was significantly inhibited by Cd2+ at 1 μM.The formation of larger protein-DNA complexes was particularly sensitive to Cd2+.The intereaction between HMG box I and DNA was completely inhibited by 10 μM of Cd2+,yet this intereaction was not inhibited by the same concentration of Ca2+. Hg2+ at 0.1μM began to cause abnormal band shifting, and protein-DNA bands disappeared to the wells of a polyacrylamide gel in the presence of 10 μM of Hg2+, reflecting that a drastic change in the conformation of HMG box I-DNA had occurred. The binding of HMG box I to DNA was slightly disturbed by As3+ at 1 μM and was significantly affected at 10 μM.Our results suggest that inhibition of the normal binding of UBF to its target DNA may be one of the mechanisms of heavy metal-induced inhbbtion of RNA synthesis.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37640
Appears in Collections:[生命科學系] 期刊論文

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