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Title: Expression of metallothionein gene during embryonic and early larval development in zebrafish
Authors: Wen-Ya Chen;Joseph Abraham Christopher John;Chih-Hung Lin;Hui-Fen Lin;Shao-Chun Wu;Cheng-Hui Lin;Chi-Yao Chang
Contributors: 國立台灣海洋大學:水產養殖系
Keywords: Zebrafish;Danio rerio;Metallothionein;Zinc;Metal-responsive element (MRE);Whole-mount in situ hybridization;Embryonic development;Promoter
Date: 2004-08
Issue Date: 2016-03-21T05:58:35Z
Publisher: Aquatic Toxicology
Abstract: Abstract:Metallothionein (Mt) has been considered as a molecular marker of metal pollution in aquatic ecosystems. Less is known about the expression of mt gene during embryogenesis. Here, we report the cloning, sequencing, and the expression pattern of mt gene during developmental stages in zebrafish. The zebrafish embryogenesis when takes place in a medium containing a dosage of 1000 μM zinc resulted in high mortality, indicating the deleterious effect of zinc on development. The zebrafish mt gene consists of three exons encoding 60 amino acids with 20 conserved cysteine residues. RT-PCR result indicates the maternal contribution of Mt transcripts. Using digoxigenin (DIG)-labeled anti-sense RNA probe, whole-mount in situ hybridization was performed to observe the expression pattern of zebrafish mt gene during embryonic and early larval stages. Stronger as well as ubiquitous expression of mt gene during early embryonic stages narrowed to specific expression after hatching. The mt promoter region contains seven copies of putative metal-responsive elements (MREs), which are shown to be important for the high level activity by deletion analysis. The expression of mt gene during embryogenesis implies its significant role on development.
Relation: 69(3),pp.215-227
Appears in Collections:[Department of Aquaculture] Periodical Articles

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