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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37546

Title: Differential messenger RNA transcription of androgen receptor and estrogen receptor in gonad in relation to the sex change in protandrous black porgy, Acanthopagrus schlegeli.
Authors: Chun-Lin He;Jin-Lien Du;Yan-Horn Lee;Yu-Shan Huang;Yoshitaka Nagahama;Ching-Fong Chang
Contributors: 國立台灣海洋大學:水產養殖系
Date: 2003-08
Issue Date: 2016-03-21T01:56:08Z
Publisher: Biol Reprod
Abstract: Abstract:Black porgy, Acanthopagrus schlegeli Bleeker, is a marine protandrous hermaphrodite fish. All are functional males at 1-2 yr of age and then become either males or females at 3 yr of age. To study the process of sex change in this species, mRNA transcripts of two estrogen receptors (ERalpha and ERbeta) and an androgen receptor (AR) were monitored. An AR cDNA was cloned and characterized. ERalpha, ERbeta, and AR were differentially transcribed in bisexual testicular and ovarian tissue according to reverse transcription polymerase chain reaction (RT-PCR) and Southern analysis. A real-time quantification PCR analysis was further developed for the measurement of AR, ERalpha, and ERbeta transcripts. ERalpha and AR transcripts were significantly more plentiful in bisexual testis than in bisexual ovary in 1(+)- and 2(+)-yr-old fish. ERalpha, ERbeta, and AR transcripts decreased in the functional testis of 3-yr-old fish. Similar levels of ERbeta and AR were detected in the ovary of sex-changed females and in functional testis of 3-yr-old males. Significantly decreased AR transcripts were found in testicular tissue of bisexual and functional male and female gonads in 3-yr-old fish as compared with 1- and 2-yr-old fish. In contrast, increased ERalpha transcripts were detected in the bisexual ovary and sex-changed ovary of 3-yr-old fish as compared with the bisexual ovary of 1- and 2-yr-old fish. The data suggest a differential sensitivity in the bisexual testicular and ovarian tissue of black porgy.
Relation: 69(2),pp.455-61
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37546
Appears in Collections:[水產養殖學系] 期刊論文

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