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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37258

Title: Monitoring and preparation of neoagaro- and agaro-oligosaccharide products by high performance anion exchange chromatography systems
Authors: Bartosz Kazłowski;Chorng Liang Pan;Yuan Tih Ko
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: Agaro-oligosaccharides;Neoagaro-oligosaccharides;Degree of polymerization;HPAEC–PAD;Isolation
Date: 2015-05
Issue Date: 2016-01-15T07:57:44Z
Publisher: Carbohydrate Polymers
Abstract: Abstract:A series of neoagaro-oligosaccharides (NAOS) were prepared by β-agarase digestion and agaro-oligosaccharides (AOS) by HCl hydrolysis from agarose with defined quantity and degree of polymerization (DP). Chain-length distribution in the crude product mixtures were monitored by two high performance anion exchange chromatography systems coupled with a pulsed amperometric detector. Method 1 utilized two separation columns: a CarboPac™ PA1 and a CarboPac™ PA100 connected in series and method 2 used the PA100 alone. Method 1 resolved the product in size ranges consisting of DP 1–46 for NAOS and DP 1–32 for AOS. Method 2 clearly resolved saccharide product sizes within DP 26. The optimized system utilizing a semi-preparative CarboPac™ PA100 column was connected with a fraction collector to isolate and quantify individually separated products. This study established systems for the preparation and qualitative and quantitative measurements as well as for the isolation of various sizes of oligomers generated from agarose.
Relation: 122,pp.351-358
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37258
Appears in Collections:[食品科學系] 期刊論文

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