English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27248/39091
Visitors : 2415710      Online Users : 71
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37213

Title: Purification and characterization of metmyoglobin reductase from ordinary muscle of blue-fin tuna
Authors: Ching-Yung Pong;Tze-Kuei Chiou;Fang-Pei Nieh;Shann-Tzong Jiang
Contributors: 國立臺灣海洋大學:食品科學系
Keywords: metmyoglobin reductase;property;purification;tuna
Date: 2000-06
Issue Date: 2016-01-14T07:11:29Z
Publisher: Fisheries Science
Abstract: Abstract:Metmyoglobin (Met-Mb) reductase from blue-fin tuna ordinary muscle was purified to electrophoretical homogeneity by ammonium sulfate fractionation, ion exchange and organomercurial agarose affinity chromatography. The molecular weight estimated by SDS-PAGE was 100 kDa. The optimal pH and temperature for the reduction of met-Mb were 7.3 and 25°C, respectively. This enzyme was very stable at pH 7.0–7.3 and 4°C–15°C. The purified enzyme was strongly activated by K+, moderately activated by Na+ and Mn2+, but not affected by Ni2+. It was moderately inhibited by Li+, NH4+, Mg2+, and Co2+. The Vmax for the reduction of met-Mb and NADH were 0.32 and 0.43 mM/min/mg, while Km were 2.3 × 10−5 and 24.4 × 10−5 M, respectively.
Relation: 66(3)
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37213
Appears in Collections:[食品科學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML93View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback