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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37165

Title: Effect of dialyzate fractions of spinach on growth of human-derived cells
Authors: Zwe-Ling Kong
Hiroki Murakami
Kazuki Shinohara
Contributors: 國立臺灣海洋大學:食品科學系
NTOU:Department of Food Science
Keywords: cell growth;human-derived cells and serum-free medium;morphology;spinach;U-937 cells
Date: 1991-10
Issue Date: 2016-01-11T02:12:21Z
Publisher: Kluwer Academic Publishers
Abstract: Abstract:Aqueous dialyzate of spinach was separated by Sephadex G-100, G-25 gel-filtrations and DEAE-cellulose ion exchange chromatography, and the effects of the fractions on growth of human-derived normal and cancer cell lines were studied. One of the fractions (SPW2) from a Sephadex G-100 gel-filtration of dialyzate promoted growth of a hybridoma cell line (HB4C5). Sephadex G-25 gel filtration of the SPW2 fraction produced four main fractions; SPW2-1, SPW2-2, SPW2-3 and SPW2-4. Among them, the SPW2-1, SPW2-3 and SPW2-4 fractions promoted the growth of a histiocytic lymphoma cell line (U-937) and hybridoma cell lines (HB4C5 and SI102). Both SPW2-3 and SPW2-4 fractions inhibited the growth of a breast cancer cell line (MCF-7). The SPW2-3 fraction, especially, was found to inhibit growth of cancer cell lines such as MCF-7, a differentiated hepatoma (HuH-7), a lung adenocarcinoma (PC-8), a lung squamous carcinoma (QG-56), and a lung anaplastic carcinoma (QG-90) more preferentially than that of normal cell lines. It was also found that a constituent of the SPW2-3 fraction caused the morphological alteration of U-937 cells in serum-free medium.
Relation: pp113-119
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37165
Appears in Collections:[食品科學系] 期刊論文

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