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Title: Overexpression of a Zebrafish ARNT2-like Factor Represses CYP1A Transcription in ZLE Cells
Authors: Wen-Der Wang
Jun-Chyi Wu
Hwei-Jan Hsu
Zwe-Ling Kong
Chin-Hwa Hu
Contributors: 國立臺灣海洋大學:食品科學系
NTOU:Department of Food Science
Date: 2000-07
Issue Date: 2016-01-04T03:31:10Z
Publisher: Springer
Abstract: Abstract:Aryl hydrocarbon receptor nuclear translocator (ARNT) factors belong to a novel basic-helix-loop-helix-PAS (bHLH-PAS) transcription factor family that controls a variety of physiological and developmental processes. In a previous study, we obtained a partial complementary DNA fragment of an ARNT2-like factor from zebrafish embryo, liver, and other tissues by reverse transcription-polymerase chain reaction. In an effort to characterize the function of this factor, we screened an embryonic complementary DNA library and obtained a complete cDNA of this ARNT2-like factor, zARNT2A. The deduced protein sequence of zARNT2A encompasses the basic-helix-loop-helix and PAS-A/B motifs and shares highest sequence similarity with the amino-terminal half of mouse ARNT2 factor. However, it lacks a carboxy-terminal transactivation motif following the PAS-A/B motifs. Transient expression of zARNT2A in cultured cells resulted in repression of TCDD-dependent CYP1A transcription. Whole-mount in situ hybridization revealed that zARNT2A is expressed in brain and pronephros at prime-5 stages. In adult fish, zARNT2A messenger RNA is transcribed in a wide range of tissues, which indicates that zARNT2A and its corresponding signal transduction mechanisms have important roles in fish development and other physiological aspects.
URI: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/37068
Appears in Collections:[食品科學系] 期刊論文

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