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|Title: ||一條根機能性萃取物滴劑提升孔雀魚 物流中之存活率|
Root Extract of I-Tiao Gung (Glycine tomentella) as Anti-Stress Drop to Increase Survival of Guppy (Poecilia reticulate) in Logistics
|Authors: ||Tzu-Yun Huang|
|Contributors: ||NTOU:Department of Food Science|
Glycine tomentella;Poecilia reticulate;anti-stress;simulated transport;logistic
|Issue Date: ||2013-10-07T02:50:30Z
|Abstract: ||觀賞魚運輸過程中，易受環境緊迫造成死亡，本研究開發天然之抗緊迫試劑，以增加孔雀魚對緊迫之耐受性。研究目標包括： (1) 設計一模式模擬運輸觀賞魚，評估孔雀魚物流中使用 GTE 滴劑對抗運輸緊迫之效果； (2) 開發抗緊迫試劑，建立使用劑量與方法； (3) 鑑定一條根乙醇萃取液 (GTE) 之機能性成分，增加孔雀魚對緊迫之耐受性； (4) 探討此滴劑長期儲存之安定性，供孔雀魚包裝運輸前處理之用，以提升在物流中之存活率。模擬孔雀魚運輸試驗中，在孔雀魚包裝水中添加 GTE 滴劑 6.25 ~ 37.5 μg/mL 魚隻存活率 (0 ~ 3%)，顯著低於控制組 (19%)；將 GTE 濃度降至 25 ~ 100 μg/L 孔雀魚運輸過程中存活率達 88 ~ 98 %，以添加 100 μg/L 組存活率為最高 (98%)，並顯著高於控制組 (60%) 及酒精組 (60%)；GTE 100 ~ 125 μg/L魚隻存活率 (89 ~ 90%) 同樣高於控制組 (82%) 及水質安定劑組 (74%)。運輸後控制組及添加水質安定劑組有水質惡化、魚體表有黴菌感染、鰓組織有充血、氯細胞增生等情形，但添加 GTE 滴劑可改善此現象。孔雀魚受緊迫後，於鰓組織中測得發炎指標 TNF-α 及 PGE2 表現量明顯上升，可作為分析小型魚之發炎指標。包裝水中添加 GTE 100 μg/L 滴劑組，其鰓組織中 TNF-α 表現量低於偵測極限，亦皆低於控制組 (3.54 ± 2.15 pg/mg protein) 及水質安定劑組 (0.90 ± 0.68 pg/mg protein)。PGE2 表現量亦以添加 GTE 100 μg/L 滴劑組 (4133 ± 714.1 pg/mg protein) 顯著低於控制組 (7667.9 ± 1405.2 pg/mg protein) 及水質安定劑組 (6245.9 ± 546.0 pg/mg protein)。其他觀賞魚中阿里、血鸚鵡及紅翅白馬包裝時添加 GTE 滴劑效果亦優於添加水質安定劑；但所使用之滴劑濃度不適用基改之斑馬魚 TK-2 (邰港螢光魚)，需大幅調降，故運輸包裝時使用 GTE 之劑量因魚種而異。GTE 抗緊迫之有效成分，以 HPLC 鑑定出 polyphenols、flavonoids、tartaric acid、p-hydroxybenzoic acid。以 GTE 作成滴劑，裝入避光塑膠罐中，分別於 25 及 40℃ 儲存 12 個月，萃取液色澤、pH、機能性成分與初始時無顯著差異 (p0.05)。|
Ornamental fish are vulnerable to stress during transportation. The aim of this study was to create a natural anti-stress drop for guppy to increase stress tolerance in transport. The main objectives are the following: (1) to design a simulated transport model to evaluate the effect of GTE (Glycine tomentella) extract drop in packing water of guppy (Poecilia reticulate); (2) to standardize the application method of using GTE drop during logistics; (3) to identify the functional compounds and the anti-stress effects of GTE; (4) to determine the storage stability and shelf- life of the GTE drop for improving the survival rate of guppy. Cumulative survival rate during simulated transportation of guppy packed in GTE drop containing 6.25-37.5 μg/mL showed 0% survival while the control group was 19%. GTE concentration was reduced to 25-100 μg/L, resulted in increased survival rate (88-98%) being higher than that of the control (60%) and the ethanol group (60%), based on equal volume of active agent added. Survival rate was highest (98%) for the GTE 100 μg/L group. GTE 100-125 μg/L groups showed survival rate of 89-90% higher than the control (82%) and the commercial drop (Aquaguard) group (74%). The 3 groups including control, ethanol and Aquaguard showed deterioration in water quality, fungal infection on the dead fish and hyperplasia of the chloride cells, fusion of secondary lamellae and hyperemia of the gill tissue in the survived ﬁsh after simulated transport. GTE drop added into packing water improved these phenomena. The pro-inflammatory responses (TNF-α and PGE2) were significantly increased in gill tissue of guppy exposed to stressors. GTE signiﬁcantly inhibited TNF-α (not detected) and PGE2 expression (4133 ± 714.1 pg/mg protein), being lower than the control (3.54 ± 2.15 and 7667.9 ± 1405.2 pg/mg protein respectively) and the Aquaguard group (0.90 ± 0.68 and 6245.9 ± 546.0 pg/mg protein respectively). Expression of TNF-α and PGE2 in gill tissue can be used as marker of inflammatory responses of small fish of which blood sample was not easy to draw. GTE drop was also effective to improve the stress tolerance of other ornamental fishes including Sciaenochromis ahli, blood parrot fish (Cichlasoma citrinellum × C. synsilum) and Metriaclima greshakei better than the Aquaguard. However, the GTE drop at the concentration used did not show positive effect in the gene modified fluorescent zebra fish TK-2. The efficacy and optimal dosage of GTE drop varies with fish species. Guppy has provided an ornamental fish model to prove that GTE can be applied as a stress-tolerance enhancing agent for the ornamental fish logistics. The anti-stress bioactive compounds of GTE were identified with HPLC. Total polyphenols, total flavonoids, tartaric acid, p-hydroxybenzoic acid were present in the GTE extract. The GTE drop stored in light-proof plastic bottles for 12 months at room temperature or 40℃ showed no significant (p>0.05) differences in color, pH and functional compounds from the initial preparation.
|Appears in Collections:||[食品科學系] 博碩士論文|
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