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Oil Production and Composition, and Biomass of Botryococcus braunii Cultured under Different Light Intensities and Nitrogen Concentrations.
|Authors: ||Ya-Chi Lin|
|Contributors: ||NTOU:Department of Aquaculture|
Botryococcus braunii;hydrocarbon;light;nitrogen;oil races
|Issue Date: ||2013-10-07T02:43:00Z
|Abstract: ||叢粒藻（Botryococcus braunii）為能夠生產大量碳氫化合物的微細藻，而被認為是一個極具潛力的可再生能源材料，故吸引了許多學者研究探討。本實驗目的為研究在不同光度與氮濃度下對叢粒藻的生物量與油脂萃取物含量之影響，以獲得能促使叢粒藻之泌油下產生生質能源的最佳條件。本研究之叢粒藻的生物量及泌油量（脂肪酸成分與碳氫化合物含量）亦被探討。結果發現，如欲培養叢粒藻來生產生質能源，則以叢粒藻NTOU種在6NB培養基及121.5-173.475 µmol m-2 S-1光度下可得到最佳生產油脂萃取物與碳氫化合物產量。相對地，叢粒藻UTEX種則以6NB培養基，在67.5-81 µmol m-2 S-1光度下可得到最佳的油脂萃取物與碳氫化合物產量。在萃取之油脂類中，叢粒藻NTOU種脂肪酸佔0.22~1.82%，其他碳氫化合物佔98.18~99.78%，而叢粒藻UTEX種脂肪酸佔0.96~3.31%，其他碳氫化合物佔96.69~99.04%，兩株叢粒藻油脂萃取物成分大部分皆為碳氫化合物。本研究依油脂萃取物的組成、培養過程藻類的顏色及型態判定叢粒藻NTOU種應屬於B race，UTEX種則屬於L race。|
Botryococcus braunii is a microalga that can produce a great quantity of hydrocarbon. It has been regarded as a potential and renewable energy resource, that attracting a lot of studies in this field. The present study tried to analysis the effects on biomass as well as oil content and composition in varied light intensities and concentrations of nitrogen. Consequently, obtained the best factors for B. braunii secrete oil for producing bio-energy. In addition, the biomass and oil contents of B. braunii (fatty acid compositions and quantity of hydrocarbon) were also studied in the present study. The results of the present study showed, that if need to produce bio-energy, therefore cultured B. braunii NTOU with 6NB medium and 121.5-173.475 µmol m-2 s-1 of light intensities were suggested, in which can obtain the best quantities of oil and hydrocarbon. In contrast, B. braunii UTEX were cultured with 6NB medium and 67.5-81 µmol m-2 s-1 light intensities can obtain the best quantities of oil and hydrocarbon. Of the oil extrgaction, fatty acids occupied 0.22~1.82%, and other hydrocarbon was 98.18~99.78% of B. braunii NTOU, while fatty acids occupied 0.96~3.31%, and other hydrocarbon was 96.69~99.04% of B. braunii UTEX. According to the algal colors and morphologies, and the oil races they produced, the B. braunii of NTOU and UTEX of the present study belong to B and L races, respectively.
|Appears in Collections:||[水產養殖學系] 博碩士論文|
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